Publication: ANTIBACTERIAL EFFICACY AND SACCHARIDE COMPOSITION OF HONEY DETERMINED BY MICROBIAL ASSAYS AND GC-MS
Loading...
Date
2026-04-16
Authors
Beaulieu, Sierra
Journal Title
Journal ISSN
Volume Title
Type
Poster
Abstract
Honey is a food product that has been used for centuries as a natural remedy. In light of growing concern regarding antibacterial resistance, honey has been investigated as an antibacterial agent. This presentation considered the efficacy of honey as an antibacterial agent and quantified biochemical properties that may allow honey to be used as an alternative therapy. To determine this, testing began by utilizing an agar disc diffusion method to determine how different types of honey inhibited the growth of bacteria, these included E. coli, P. aeruginosa, and B. subtilis. No significant differences were observed between the two honey types, where a raw honey product and a commercially available honey were each tested. Further analysis was conducted to determine a minimum inhibitory concentration of honey, where each honey type was diluted 10-fold in a 96-well plate containing an E. coli and B. subtilis culture. Further testing was performed to deduce what biochemical properties contribute to the antimicrobial characteristics of honey. A two-step derivatization process allowed gas chromatography-mass spectrometry (GC-MS) to be used as the main method to identify saccharides in honey samples by utilizing retention index values compared to a set standard. Further testing was performed to calculate the exact concentrations of fructose, glucose, and sucrose in each honey sample. Additional testing on the heavy metal contents of honey was also carried out as a means to further compare the raw and commercially available honeys, along with their antibacterial efficacy. Together, these methods help determine the value of honey as an alternative therapy. This presentation highlights the need for further research on alternative antibacterials and provides a foundation for methods and possible antimicrobial agents.