Publication: PAPAIN-CATALYZED SYNTHESIS OF ALTERNATING ARGININE-TRYPTOPHAN PEPTIDES FOR ANTIMICRIOBIAL APPLICATIONS
| dc.contributor.author | Nichols, Kaleb | |
| dc.contributor.author | Edson, Cody | |
| dc.date.accessioned | 2026-04-22T19:29:14Z | |
| dc.date.issued | 2026-04-16 | |
| dc.description.abstract | Antimicrobial peptides represent a promising strategy for combating multidrug resistant pathogens. However, the conventional synthesis strategy using Solid Phase Peptide Synthesis (SPPS) relies on harsh reagents, extensive solvent use and costly, multi-step protocols. In this study, we investigate a greener and more efficient approach to synthesizing peptides using Protease-Catalyzed Peptide Synthesis (PCPS) to generate short arginine-tryptophan (Arg-Trp) peptides, molecules known to possess antimicrobial properties. We examine the enzymatic coupling of Arg-Trp dipeptides using papain, a well-documented protease, as a biocatalyst under mild conditions, promoting peptide bond formation through aminolysis rather than hydrolysis. The reaction products are characterized by MALDI-TOF mass spectrometry to assess peptide formation and composition. This approach aims to provide a cost-effective and environmentally sustainable alternative to traditional peptide synthesis, with findings that will inform future studies on alternative syntheses and antimicrobial efficacy of Arg–Trp peptides produced via PCPS. | |
| dc.identifier.uri | https://dspace.husson.edu/handle/20.500.14298/1114 | |
| dc.language.iso | en_US | |
| dc.title | PAPAIN-CATALYZED SYNTHESIS OF ALTERNATING ARGININE-TRYPTOPHAN PEPTIDES FOR ANTIMICRIOBIAL APPLICATIONS | |
| dc.type | Poster | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | d3e89195-c9f5-4e19-926c-52273d4dbc4c | |
| relation.isAuthorOfPublication.latestForDiscovery | d3e89195-c9f5-4e19-926c-52273d4dbc4c |